7C20

Crystal structure of Rabies virus (Nishigahara strain) phosphoprotein C-terminal domain (K214A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-30
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9
Spacegroup name	P 41 21 2
Unit cell lengths	51.265, 51.265, 90.108
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.250 - 3.000
R-factor	0.2094
Rwork	0.207
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vyi
RMSD bond length	0.003
RMSD bond angle	0.545
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	3.290
High resolution limit [Å]	3.000	3.000
Rmeas	0.158	0.635
Rpim	0.056	0.164
Number of reflections	2984	2384
<I/σ(I)>	5	1.3
Completeness [%]	100.0	100
Redundancy	13.6	14.7
CC(1/2)	0.998	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293	10 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 3mM EDTA, 0.05% Tween20