7C1X

Unliganded structure of Pseudouridine kinase (PUKI) from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-24
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	80.686, 48.352, 92.751
Unit cell angles	90.00, 109.14, 90.00

Refinement procedure

Resolution	29.932 - 2.389
R-factor	0.210811925093
Rwork	0.208
R-free	0.24225
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.893
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.380	2.400
Number of reflections	26768	781
<I/σ(I)>	13.4
Completeness [%]	99.2
Redundancy	3.4
CC(1/2)	0.973	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1M tri sodium citrate, 22%PEG 3350