7C1U

Crystal structure of the starter condensation domain of rhizomide synthetase RzmA mutant H140V/R148A in a "product-released" conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	52.564, 58.784, 134.058
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.461 - 1.400
R-factor	0.1463
Rwork	0.145
R-free	0.17780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c1h
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.420
High resolution limit [Å]	1.400	3.800	1.400
Rmerge	0.056	0.035	0.897
Rmeas	0.058	0.037	0.936
Rpim	0.016	0.010	0.260
Total number of observations	1058554
Number of reflections	80924	4439	3784
<I/σ(I)>	7.6
Completeness [%]	97.9	99.6	92.9
Redundancy	13.1	12.6	11.8
CC(1/2)		0.999	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	289	BIS-TRIS propane, PEG 6000