7C1S
Crystal structure of the starter condensation domain of rhizomide synthetase RzmA mutant H140A/R148A in complex with C8-CoA and Leu-SNAC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-31 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.828, 72.386, 108.582 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.115 - 2.586 |
R-factor | 0.2013 |
Rwork | 0.196 |
R-free | 0.25240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7c1h |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.620 |
High resolution limit [Å] | 2.580 | 7.000 | 2.580 |
Rmerge | 0.152 | 0.063 | 0.762 |
Rmeas | 0.158 | 0.066 | 0.798 |
Rpim | 0.044 | 0.019 | 0.235 |
Total number of observations | 172673 | ||
Number of reflections | 13590 | 769 | 678 |
<I/σ(I)> | 4.6 | ||
Completeness [%] | 99.9 | 99.6 | 100 |
Redundancy | 12.7 | 11.2 | 11.3 |
CC(1/2) | 0.998 | 0.875 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 289 | BIS-TRIS propane, PEG 6000 |