7C1P

Crystal structure of the starter condensation domain of the rhizomide synthetase RzmA mutant H140V, R148A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-19
Detector	PSI PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	60.636, 89.651, 98.067
Unit cell angles	66.89, 90.02, 90.25

Refinement procedure

Resolution	48.956 - 2.600
R-factor	0.2328
Rwork	0.230
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c1h
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.589	7.050	2.600
Rmerge	0.044	0.029	0.363
Rmeas	0.051	0.034	0.424
Rpim	0.027	0.018	0.217
Number of reflections	55871	2783	2871
<I/σ(I)>	10.2
Completeness [%]	94.8	94.3	95.5
Redundancy	3.6	3.6	3.6
CC(1/2)		0.998	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	289	Citric acid, BIS-TRIS propane, PEG 3350