7C1K

Crystal structure of the starter condensation domain of rhizomide synthetase RzmA mutant R148A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-19
Detector	SIEMENS-NICOLET
Wavelength(s)	0.976
Spacegroup name	P 1
Unit cell lengths	59.965, 89.701, 98.345
Unit cell angles	113.22, 90.09, 89.92

Refinement procedure

Resolution	39.121 - 2.755
R-factor	0.2385
Rwork	0.237
R-free	0.27450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c1h
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.755	7.590	2.800
Rmerge	0.067	0.034	0.366
Rmeas	0.078	0.040	0.434
Rpim	0.041	0.021	0.230
Number of reflections	45676	2279	2135
<I/σ(I)>	7.9
Completeness [%]	94.8	95	88.7
Redundancy	3.6	3.7	3.4
CC(1/2)		0.997	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	289	Citric acid, BIS-TRIS propane, PEG 3350