7C1H

Crystal structure of the starter condensation domain of rhizomide synthetase RzmA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-24
Detector	PSI PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	60.051, 88.764, 97.883
Unit cell angles	67.30, 89.90, 89.92

Refinement procedure

Resolution	49.974 - 2.300
R-factor	0.2297
Rwork	0.227
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jn3
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.296	6.240	2.300
Rmerge	0.031	0.020	0.505
Rmeas	0.036	0.023	0.591
Rpim	0.019	0.012	0.306
Number of reflections	78613	3821	3971
<I/σ(I)>	11.6
Completeness [%]	94.7	91.9	96.5
Redundancy	3.6	3.7	3.6
CC(1/2)		0.998	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	298	Citric acid, BIS-TRIS propane, PEG 3350