7C0N
Crystal structure of a self-assembling galactosylated peptide homodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2019-06-08 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.54191 |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 72.837, 72.837, 72.837 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.752 - 1.552 |
Rwork | 0.234 |
R-free | 0.24950 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.078 | 0.369 |
Number of reflections | 2694 | 256 |
<I/σ(I)> | 54.2 | 17 |
Completeness [%] | 100.0 | |
Redundancy | 72.6 | 63 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 40%(w/v) PEG 1000, 0.1 M Sodium phosphate dibasic/Citric acid(pH 4.2), 0.2 M Lithium sulfate |