7C0D
Crystal structure of Azospirillum brasilense L-2-keto-3-deoxyarabonate dehydratase (Hydroxypyruvate-bound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.377, 88.471, 143.700 |
| Unit cell angles | 84.83, 86.00, 73.21 |
Refinement procedure
| Resolution | 48.806 - 1.600 |
| R-factor | 0.1716 |
| Rwork | 0.170 |
| R-free | 0.19860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.846 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.810 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.083 | 0.506 |
| Rmeas | 0.093 | 0.560 |
| Rpim | 0.040 | 0.238 |
| Number of reflections | 459937 | 22165 |
| <I/σ(I)> | 14.3 | 4.4 |
| Completeness [%] | 97.1 | 94.1 |
| Redundancy | 5.3 | 5.4 |
| CC(1/2) | 0.996 | 0.913 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M magnesium chloride, 0.1 M Tris-HCl, 22-27% PEG 3350 |
