7C09

Structure of lysozyme obtained in SSRF using serial crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-12-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	78.000, 78.000, 38.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.577 - 2.200
R-factor	0.1828
Rwork	0.178
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
Data reduction software	MOSFLM
Data scaling software	CrystFEL (8.0)
Phasing software	PHENIX (1.14_3260)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.580	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	6326	1131
<I/σ(I)>	4.5
Completeness [%]	99.9
Redundancy	11.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	NaCl