7C07

Crystal structure of yeast U2AF1 complex bound to 5'-AAGGU RNA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-24
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	94.487, 256.947, 94.591
Unit cell angles	90.00, 100.75, 90.00

Refinement procedure

Resolution	48.170 - 3.200
R-factor	0.2563
Rwork	0.255
R-free	0.28540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yh8
RMSD bond length	0.002
RMSD bond angle	0.447
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.170	3.270
High resolution limit [Å]	3.200	3.200
Rmerge	0.117	0.802
Number of reflections	72802	4522
<I/σ(I)>	10.2	2.1
Completeness [%]	100.0	100
Redundancy	4.9	4.8
CC(1/2)	0.996	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M NaCitrate, 10% PEG 35000