7C03
Crystal structure of POLArISact(T57S), genetically encoded probe for fluorescent polarization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-09-07 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 78.130, 113.590, 132.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.202 - 2.501 |
R-factor | 0.2165 |
Rwork | 0.214 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3evp |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER (2.8.1) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 20785 | 45440 |
<I/σ(I)> | 13.3 | |
Completeness [%] | 99.9 | |
Redundancy | 7.1 | |
CC(1/2) | 0.912 | 0.644 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M sodium acetate (pH4.5-5.0), 6-8% (v/v) PEG 4000 |