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7C03

Crystal structure of POLArISact(T57S), genetically encoded probe for fluorescent polarization

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X6A
Synchrotron siteNSLS
BeamlineX6A
Temperature [K]100
Detector technologyCCD
Collection date2018-09-07
DetectorADSC QUANTUM 210
Wavelength(s)1
Spacegroup nameI 21 21 21
Unit cell lengths78.130, 113.590, 132.700
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution46.202 - 2.501
R-factor0.2165
Rwork0.214
R-free0.23790
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3evp
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER (2.8.1)
Refinement softwarePHENIX (1.13_2998)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.640
High resolution limit [Å]2.5002.500
Number of reflections2078545440
<I/σ(I)>13.3
Completeness [%]99.9
Redundancy7.1
CC(1/2)0.9120.644
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP2930.1M sodium acetate (pH4.5-5.0), 6-8% (v/v) PEG 4000

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