7C00
Crystal structure of the SRCR domain of human SCARA5.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.167, 48.802, 70.771 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.647 - 1.700 |
R-factor | 0.1597 |
Rwork | 0.158 |
R-free | 0.17510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SRCR domain of mouse SCARA5 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.650 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.082 | 0.393 |
Number of reflections | 16428 | 1606 |
<I/σ(I)> | 29.03 | |
Completeness [%] | 99.6 | |
Redundancy | 12.7 | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | 40% (w/v) polyethylene glycol 400, 0.2M sodium chloride, 0.1M Tacsimate (pH 8.0) |