7BZM
Crystal structure of rice Os3BGlu7 with glucoimidazole
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-02 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.402, 101.557, 127.458 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.300 |
R-factor | 0.1786 |
Rwork | 0.177 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rgl |
RMSD bond length | 0.008 |
RMSD bond angle | 1.265 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 47470 | 2303 |
<I/σ(I)> | 17.3 | 6.6 |
Completeness [%] | 99.9 | |
Redundancy | 6.9 | |
CC(1/2) | 0.998 | 0.947 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 288 | 23% PEG MME 5000, 0.17 M AMMONIUM SULFATE, 0.1M MES, PH 6.7 |