7BZK
Crystal structure of ferredoxin: thioredoxin reductase and thioredoxin y1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.985, 60.762, 61.056 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.084 - 1.593 |
R-factor | 0.171 |
Rwork | 0.170 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pu9 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.084 | 1.651 |
High resolution limit [Å] | 1.593 | 1.593 |
Rmerge | 0.120 | 0.761 |
Rmeas | 0.130 | 0.831 |
Rpim | 0.050 | 0.331 |
Number of reflections | 27925 | 2737 |
<I/σ(I)> | 10.4 | |
Completeness [%] | 99.9 | 99.56 |
Redundancy | 6.6 | 6.2 |
CC(1/2) | 0.995 | 0.861 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293.15 | 100 mM Magnesium Citric acid pH 3.5 5% 2-propanol 5-9% PEG 3,350 |