7BZD
Structure of Bacillus subtilis HxlR, wild type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.161, 60.772, 70.681 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.081 - 2.612 |
| R-factor | 0.2438 |
| Rwork | 0.241 |
| R-free | 0.27060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a5n |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.194 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260: ???) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.065 | 0.816 |
| Number of reflections | 3670 | 188 |
| <I/σ(I)> | 28.7 | 3.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium acetate trihydrate pH = 4.6, 1.5 M ammonium chloride, 10 mM DTT |
