7BZC
Crystal structure of plant sesterterpene synthase AtTPS18 complexed with farnesyl thiolodiphosphate (FSPP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9798 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 64.388, 81.537, 125.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.445 - 2.303 |
R-factor | 0.2043 |
Rwork | 0.202 |
R-free | 0.24340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eat |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.445 | 2.385 |
High resolution limit [Å] | 2.300 | 2.303 |
Number of reflections | 38230 | 2080 |
<I/σ(I)> | 20 | |
Completeness [%] | 99.7 | |
Redundancy | 10.8 | |
CC(1/2) | 0.998 | 0.708 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% (w/v) PEG 8000, 0.04M potassium phosphate (monobasic), 20% (v/v) glycerol |