7BYV

Crystal structure of exo-beta-1,3-galactanase from Phanerochaete chrysosporium Pc1,3Gal43A E208Q with beta-1,3-galactotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	CCD
Collection date	2009-10-22
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	66.080, 50.412, 75.698
Unit cell angles	90.00, 111.33, 90.00

Refinement procedure

Resolution	29.790 - 2.500
R-factor	0.1701
Rwork	0.166
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bys
RMSD bond length	0.008
RMSD bond angle	0.931
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3546)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.499	2.500
Number of reflections	16007	702
<I/σ(I)>	13.5	2.7
Completeness [%]	96.2	83
Redundancy	4.4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	16 % (w/v) polyethylene glycol (PEG) 10000, 95 mM ammonium sulfate, 95 mM bis-tris, 4.8 % glycerol, 10 mM beta-1,3-galactotriose