7BYS

Crystal structure of exo-beta-1,3-galactanase from Phanerochaete chrysosporium Pc1,3Gal43A apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2008-02-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.90646
Spacegroup name	P 1
Unit cell lengths	40.470, 66.262, 74.021
Unit cell angles	72.03, 84.68, 82.15

Refinement procedure

Resolution	8.000 - 1.400
R-factor	0.1562
Rwork	0.155
R-free	0.18560
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.16_356)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	136692	12747
<I/σ(I)>	22.4	2.8
Completeness [%]	95.6	89
Redundancy	1.9	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	2.1 M ammonium sulfate, 0.1 M citrate buffer pH 5.5.
2	VAPOR DIFFUSION	5.5	293	16% (w/v) polyethylene glycol (PEG) 10000 95 mM ammonium sulfate 95 mM bis-tris 4.8% (v/v) glycerol