7BYE

Toxin-antitoxin complex from klebsiella pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	193
Detector technology	CCD
Collection date	2018-05-31
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9794
Spacegroup name	P 1
Unit cell lengths	41.558, 45.501, 69.952
Unit cell angles	102.06, 93.60, 116.36

Refinement procedure

Resolution	50.000 - 2.300
Rwork	0.198
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.184
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rpim	0.067	0.111
Number of reflections	17681	909
<I/σ(I)>	18.9
Completeness [%]	89.5
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tris