7BY3
Toxin-antitoxin complex from klebsiella pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2017-09-28 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 110.435, 79.019, 59.609 |
Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
Rwork | 0.244 |
R-free | 0.27923 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.220 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.080 |
High resolution limit [Å] | 2.000 | 2.040 |
Rpim | 0.037 | 0.267 |
Number of reflections | 31667 | 1644 |
<I/σ(I)> | 48.3 | |
Completeness [%] | 96.8 | |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Tris |