7BY2

Toxin-antitoxin complex from Klebsiella pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	193
Detector technology	CCD
Collection date	2017-11-03
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9794
Spacegroup name	C 2 2 21
Unit cell lengths	79.200, 110.323, 59.703
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.600
Rwork	0.269
R-free	0.32010
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.481
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rpim	0.054	0.063
Number of reflections	8245	397
<I/σ(I)>	57.8
Completeness [%]	99.9
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tris