7BXZ

Crystal structure of the aminoglycoside 6'-N-acetyltransferase from Enterococcus faecium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-07-14
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.977872
Spacegroup name	C 1 2 1
Unit cell lengths	68.426, 90.317, 102.151
Unit cell angles	90.00, 96.08, 90.00

Refinement procedure

Resolution	29.895 - 2.502
R-factor	0.2278
Rwork	0.226
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g9p
Data reduction software	SCALEPACK
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.540
High resolution limit [Å]	2.500	6.760	2.500
Rmerge	0.064	0.030	0.472
Rmeas	0.069	0.032	0.512
Rpim	0.026	0.012	0.196
Total number of observations	148028
Number of reflections	21196	1107	1041
<I/σ(I)>	5.3
Completeness [%]	98.9	99.6	97.7
Redundancy	7	7.4	6.7
CC(1/2)		0.999	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM HEPES-Na (pH 7.0), 10 mM magnesium chloride, 1.6 M ammonium sulfate