7BXY

mRNA interferase from Bacillus cereus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	193
Detector technology	CCD
Collection date	2019-10-30
Detector	RAYONIX MX300HE
Wavelength(s)	0.899995
Spacegroup name	P 31 2 1
Unit cell lengths	60.648, 60.648, 76.247
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.250 - 1.980
R-factor	0.1931
Rwork	0.189
R-free	0.22540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.108
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	1.980	1.980
Number of reflections	12876	3402
<I/σ(I)>	10.74
Completeness [%]	99.3
Redundancy	10.57
CC(1/2)	0.997	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tris