7BXV
11A1 antibody-peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.639, 123.774, 45.692 |
| Unit cell angles | 90.00, 99.74, 90.00 |
Refinement procedure
| Resolution | 45.074 - 1.750 |
| Rwork | 0.166 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mnu |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.275 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.063 | 0.135 |
| Rmeas | 0.068 | 0.147 |
| Rpim | 0.026 | 0.056 |
| Number of reflections | 44761 | 2456 |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3,350, NaCl |
