7BXH

MavC-Lpg2149 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-06-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	58.651, 62.817, 138.391
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.182 - 2.700
R-factor	0.2079
Rwork	0.205
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tsc 5dpo
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.760
High resolution limit [Å]	2.700	2.700
Rmerge	0.125	0.524
Number of reflections	14489	14489
<I/σ(I)>	15.5
Completeness [%]	99.8
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M sodium acetate, pH 4.0, 10% PEG4000