7BXF

MvcA-Lpg2149 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-10-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	136.998, 81.229, 54.253
Unit cell angles	90.00, 95.09, 90.00

Refinement procedure

Resolution	24.821 - 2.700
R-factor	0.2166
Rwork	0.214
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5suj 5dpo
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.760
High resolution limit [Å]	2.700	2.700
Rmerge	0.064
Number of reflections	15703	1071
<I/σ(I)>	21.6
Completeness [%]	96.7
Redundancy	5.3
CC(1/2)		0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	2% Tacsimate, pH 4.0, 0.1 M 426 sodium acecate, pH 4.6, 16% PEG3350