7BX1

Crystal structure of RTT109 from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	77.36
Detector technology	CCD
Collection date	2015-04-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.975
Spacegroup name	P 1 21 1
Unit cell lengths	51.317, 69.422, 54.486
Unit cell angles	90.00, 114.40, 90.00

Refinement procedure

Resolution	49.620 - 1.580
R-factor	0.183
Rwork	0.182
R-free	0.19450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.706
Data scaling software	HKL-2000 (v1.0)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.580	1.580
Number of reflections	331312	331312
<I/σ(I)>	3.8
Completeness [%]	97.9
Redundancy	7
CC(1/2)	0.990	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289.15	0.1 M Bis-Tris pH 6.0, 15~20% PEG 3350