7BWX

Crystal structure of ice-binding protein from an Antarctic ascomycete, Antarctomyces psychrotrophicus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9800
Spacegroup name	P 21 21 2
Unit cell lengths	86.427, 207.281, 100.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.620 - 1.904
R-factor	0.1366
Rwork	0.135
R-free	0.16410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wp9
RMSD bond length	0.013
RMSD bond angle	1.695
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.620	2.010
High resolution limit [Å]	1.900	1.900
Rmerge	0.107	0.467
Rpim	0.045	0.197
Number of reflections	141990	20446
<I/σ(I)>	12.9	4.2
Completeness [%]	99.9	99.6
Redundancy	6.7	6.6
CC(1/2)	0.997	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	293	0.1 M HEPES-NaOH pH 8.6, 0.95 M ammonium sulfate and 0.1 M lithium sulfate