7BWK

Structure of DotL(656-783)-IcmS-IcmW-LvgA-VpdB(461-590) derived from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-25
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	188.992, 188.992, 170.252
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.248 - 2.801
R-factor	0.2014
Rwork	0.199
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x90
RMSD bond length	0.009
RMSD bond angle	1.014
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.106	0.039	0.311
Rmeas	0.109	0.040	0.331
Rpim	0.026	0.008	0.109
Total number of observations	1165282
Number of reflections	75746	4102	3721
<I/σ(I)>	4.3
Completeness [%]	99.5	99.5	98.7
Redundancy	15.4	23.2	7.7
CC(1/2)		1.000	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	50 mM sodium cacodylate (pH 7.0) 1.8 M ammonium sulfate