7BWK
Structure of DotL(656-783)-IcmS-IcmW-LvgA-VpdB(461-590) derived from Legionella pneumophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-11-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 188.992, 188.992, 170.252 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.248 - 2.801 |
R-factor | 0.2014 |
Rwork | 0.199 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5x90 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.014 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
Rmerge | 0.106 | 0.039 | 0.311 |
Rmeas | 0.109 | 0.040 | 0.331 |
Rpim | 0.026 | 0.008 | 0.109 |
Total number of observations | 1165282 | ||
Number of reflections | 75746 | 4102 | 3721 |
<I/σ(I)> | 4.3 | ||
Completeness [%] | 99.5 | 99.5 | 98.7 |
Redundancy | 15.4 | 23.2 | 7.7 |
CC(1/2) | 1.000 | 0.557 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 50 mM sodium cacodylate (pH 7.0) 1.8 M ammonium sulfate |