7BWH

Soluble cytochrome b5 from Ramazzottius varieornatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9000
Spacegroup name	P 2 21 21
Unit cell lengths	34.004, 37.495, 77.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.800 - 1.400
Rwork	0.147
R-free	0.17100
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.449
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (CCP4Interface 7.0.078)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.800	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.036	0.451
Rmeas	0.039	0.493
Rpim	0.016	0.196
Number of reflections	20165	965
<I/σ(I)>	24	3.9
Completeness [%]	99.7	100
Redundancy	6.3	6.2
CC(1/2)	0.999	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	26 mg/mL protein, 22% (w/v) PEG 4000, and 50 mM HEPES buffer pH 7.5