7BWG

A Glycoside Hydrolase Family 20 beta-N-Acetylglucosaminidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9789
Spacegroup name	P 1 21 1
Unit cell lengths	78.035, 81.928, 79.418
Unit cell angles	90.00, 94.08, 90.00

Refinement procedure

Resolution	57.610 - 2.850
R-factor	0.2942
Rwork	0.292
R-free	0.33450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hp4
RMSD bond length	0.003
RMSD bond angle	0.642
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.610	2.950
High resolution limit [Å]	2.848	2.848
Rmerge	0.229	0.773
Rmeas	0.252	0.845
Number of reflections	23477	2334
<I/σ(I)>	8.19	2.62
Completeness [%]	100.0	100
Redundancy	6.1
CC(1/2)	0.982	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M MES pH 6.5, 10% (V/V) Dioxane, 1.6 M (NH4)2SO4