7BWF
YoeB-YefM complex from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-31 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97942 |
Spacegroup name | P 31 |
Unit cell lengths | 67.849, 67.849, 69.356 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.832 - 1.700 |
R-factor | 0.207 |
Rwork | 0.206 |
R-free | 0.22960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a6q |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.069 | 0.611 |
Number of reflections | 38302 | 1944 |
<I/σ(I)> | 26.9 | 2.3 |
Completeness [%] | 97.4 | 99 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.08 M sodium chloride, 0.02 M magnesium chloride hexahydrate, 0.04 M sodium cacodylate trihydrate pH 7.0, 40% v/v (+/-)-2-methyl-2,4-pentanediol, and 0.012 M spermine tetrahydrochloride |