7BWB
Bombyx mori GH32 beta-fructofuranosidase BmSUC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 94.752, 119.308, 100.275 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.872 - 1.800 |
Rwork | 0.168 |
R-free | 0.21310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uyp |
RMSD bond length | 0.009 |
RMSD bond angle | 1.523 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MrBUMP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.051 | 0.607 |
Number of reflections | 52834 | 7635 |
<I/σ(I)> | 17 | 2.5 |
Completeness [%] | 99.9 | |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 13% PEG 3350, 40 mM citric acid, 60 mM Bis-Tris propane |