7BVT

Crystal structure of cyclic alpha-maltosyl-1,6-maltose binding protein from Arthrobacter globiformis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-18
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	92.077, 92.077, 161.584
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.670 - 1.470
R-factor	0.1859
Rwork	0.185
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ci5
RMSD bond length	0.013
RMSD bond angle	1.795
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.040	1.500
High resolution limit [Å]	1.470	1.470
Rmerge	0.093	1.794
Rpim	0.021	0.595
Number of reflections	68976	32216
<I/σ(I)>	15.7	1.2
Completeness [%]	99.5	95.8
Redundancy	19.6	10
CC(1/2)	0.981	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris-HCl (pH 8.5), 2.0 M ammonium sulfate