7BVS

DfgA-DfgB complex apo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-22
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 64 2 2
Unit cell lengths	138.213, 138.213, 227.001
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.240 - 2.850
R-factor	0.2007
Rwork	0.198
R-free	0.23330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dre
RMSD bond length	0.009
RMSD bond angle	0.994
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.940	3.000
High resolution limit [Å]	2.850	2.850
Rmerge	0.113	0.615
Number of reflections	30686	4392
<I/σ(I)>	24.3	5.8
Completeness [%]	100.0
Redundancy	20.1
CC(1/2)	0.999	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		313	100 mM Tris-HCl (pH8.5), 1190 mM (NH4)2SO4