7BV3

Crystal structure of a ugt transferase from Siraitia grosvenorii in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	197.520, 63.710, 93.800
Unit cell angles	90.00, 101.65, 90.00

Refinement procedure

Resolution	24.220 - 1.850
R-factor	0.1747
Rwork	0.174
R-free	0.19570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2acw
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.920
High resolution limit [Å]	1.850	3.980	1.850
Rmerge	0.062	0.032	0.728
Rmeas	0.068	0.035	0.796
Rpim	0.026	0.014	0.317
Total number of observations	651819
Number of reflections	98386	10049	9810
<I/σ(I)>	8.8
Completeness [%]	99.9	99.7	99.9
Redundancy	6.6	6.5	6.2
CC(1/2)		0.999	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	PEG8000, MgAc