7BUW

Eucommia ulmoides TPT3 mutant -C94Y/A95F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-30
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	84.253, 84.253, 181.189
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.530 - 3.300
R-factor	0.2556
Rwork	0.253
R-free	0.31040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kk2
RMSD bond length	0.004
RMSD bond angle	0.777
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.530	3.418
High resolution limit [Å]	3.300	3.300
Number of reflections	11760	1137
<I/σ(I)>	12.2
Completeness [%]	99.9
Redundancy	21.1
CC(1/2)	0.996	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	CHES, PEG 8000, Risedronate, MgCl2