7BUP
Crystal structure of PCNA from pathogenic yeast Candida albicans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2017-05-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.520, 78.910, 150.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.170 - 2.700 |
R-factor | 0.2215 |
Rwork | 0.219 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yhr |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | Aimless (0.5.29) |
Phasing software | MOLREP (11.5.06) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.170 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.031 | 0.244 |
Rmeas | 0.044 | 0.345 |
Rpim | 0.031 | 0.244 |
Number of reflections | 26417 | 2611 |
<I/σ(I)> | 2.8 | 2.8 |
Completeness [%] | 100.0 | 99.96 |
Redundancy | 2 | 2 |
CC(1/2) | 0.999 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20 % V/V Glycerol 24 % PEG 1500 |