7BUH
Reduced ferredoxin of carbazole 1,9a-dioxygenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2016-07-20 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.045, 67.725, 45.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.340 - 1.790 |
R-factor | 0.1594 |
Rwork | 0.157 |
R-free | 0.20110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vck |
RMSD bond length | 0.010 |
RMSD bond angle | 1.825 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.450 | 1.830 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.150 | 0.829 |
Number of reflections | 9143 | 532 |
<I/σ(I)> | 29.6 | |
Completeness [%] | 99.9 | |
Redundancy | 26.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Buffer: 5 mM Tris pH 7.5, Reservior: 0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.1, 30% w/v Polyethylene glycol 4000 |