7BTM
SDR protein/resistance protein NapW
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 117.149, 134.438, 117.216 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 41.332 - 2.083 |
| R-factor | 0.215636045666 |
| Rwork | 0.210 |
| R-free | 0.24910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qq5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.930 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 216044 | 10828 |
| <I/σ(I)> | 10.778 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.990 | 0.725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1.2 M Ammonium sulfate, 0.1 M BIS-TRIS propane, 0.1 M Betaine hydrochloride |
