7BT9
Mevo lectin complex with alpha-mannose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 168.849, 168.849, 193.639 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 84.567 - 2.450 |
Rwork | 0.242 |
R-free | 0.28370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bsm |
RMSD bond length | 0.012 |
RMSD bond angle | 1.891 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.820 | 2.580 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.220 | |
Number of reflections | 101188 | 13609 |
<I/σ(I)> | 6.1 | 1.5 |
Completeness [%] | 98.4 | |
Redundancy | 10.7 | |
CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6 | 296 | 6%(v/v) Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25%(w/v) polyethylene glycol 4000 |