7BSX

SDR protein NapW-NADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	116.420, 116.040, 134.760
Unit cell angles	90.00, 90.04, 90.00

Refinement procedure

Resolution	58.270 - 2.000
R-factor	0.178754056467
Rwork	0.178
R-free	0.20168
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qq5
RMSD bond length	0.007
RMSD bond angle	1.160
Data reduction software	xia2
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.270	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.082	0.846
Number of reflections	238915	17659
<I/σ(I)>	12.9
Completeness [%]	99.0
Redundancy	6.8
CC(1/2)	0.999	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	Ammonium sulfate, BIS-TRIS propane