7BSB

Mevo lectin- Native form-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-12-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	169.450, 169.450, 192.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.599 - 2.450
Rwork	0.231
R-free	0.27040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zno
RMSD bond length	0.013
RMSD bond angle	1.717
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.120	2.580
High resolution limit [Å]	2.450	2.450
Rmerge	0.140
Number of reflections	102790	14786
<I/σ(I)>	11.8	2.2
Completeness [%]	99.9	99.8
Redundancy	12.7	10
CC(1/2)	0.998	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		296	6%(v/v) Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25%(w/v) polyethylene glycol 4000