7BSB
Mevo lectin- Native form-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-12-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 169.450, 169.450, 192.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.599 - 2.450 |
| Rwork | 0.231 |
| R-free | 0.27040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zno |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.717 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.120 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.140 | |
| Number of reflections | 102790 | 14786 |
| <I/σ(I)> | 11.8 | 2.2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 12.7 | 10 |
| CC(1/2) | 0.998 | 0.828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 296 | 6%(v/v) Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25%(w/v) polyethylene glycol 4000 |
