7BRQ
Crystal structure of human FAM134B LIR fused to human GABARAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2017-10-06 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 |
Unit cell lengths | 81.892, 81.892, 33.324 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.946 - 1.404 |
R-factor | 0.1922 |
Rwork | 0.192 |
R-free | 0.20610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gnu |
RMSD bond length | 0.006 |
RMSD bond angle | 0.790 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (dev_2450) |
Refinement software | PHENIX (dev_2450) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.950 | 1.454 |
High resolution limit [Å] | 1.404 | 1.404 |
Rmerge | 0.074 | 0.631 |
Rmeas | 0.078 | 0.683 |
Rpim | 0.025 | 0.254 |
Number of reflections | 24609 | 2058 |
<I/σ(I)> | 18.8 | 2.68 |
Completeness [%] | 97.7 | 82.68 |
Redundancy | 9.7 | 6.5 |
CC(1/2) | 0.997 | 0.755 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 1.91 M ammonium sulfate, 0.1 M sodium citrate pH 5.5, 0.18 M potassium sodium tartrate |