7BRM
Architecture of curli complex
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TECNAI ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K2 BASE (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Number of Particle | 194713 |
| Software | RELION,PHENIX,SerialEM,CTFFIND |
| Symmetry | C9 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
