7BRK

Atrial Natriuretic Peptide Receptor complexed with deletion mutant of human Atrial Natriuretic Peptide[5-27]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-28
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 61
Unit cell lengths	99.419, 99.419, 259.666
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.106 - 2.850
R-factor	0.1778
Rwork	0.176
R-free	0.23090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dp4
RMSD bond length	0.006
RMSD bond angle	0.800
Data reduction software	HKL-2000 (716.1)
Data scaling software	HKL-2000 (716.1)
Phasing software	MOLREP (11.2.08)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.930
High resolution limit [Å]	2.850	2.850
Rmerge	0.078	0.243
Number of reflections	30557	2829
<I/σ(I)>	26.4	5.3
Completeness [%]	90.2
Redundancy	15.4
CC(1/2)	0.990	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	Sodium malonate, MES