7BRJ

Atrial Natriuretic Peptide Receptor complexed with deletion mutant of human Atrial Natriuretic Peptide[7-28]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-28
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 61
Unit cell lengths	99.793, 99.793, 260.397
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.258 - 2.700
R-factor	0.194
Rwork	0.193
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dp4
RMSD bond length	0.006
RMSD bond angle	0.779
Data reduction software	HKL-2000 (716.1)
Data scaling software	HKL-2000 (716.1)
Phasing software	MOLREP (11.2.08)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.780
High resolution limit [Å]	2.700	2.700
Rmerge	0.031	0.307
Number of reflections	39908	3316
<I/σ(I)>	23.5	3.5
Completeness [%]	99.3
Redundancy	10.9
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	Sodium malonate, MES