7BRG
Atrial Natriuretic Peptide Receptor complexed with rat Atrial Natriuretic Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 61 |
Unit cell lengths | 100.234, 100.234, 261.903 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.916 - 2.450 |
R-factor | 0.194 |
Rwork | 0.193 |
R-free | 0.24920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dp4 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.785 |
Data reduction software | HKL-2000 (716.1) |
Data scaling software | HKL-2000 (716.1) |
Phasing software | MOLREP (11.2.08) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.520 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.060 | 0.355 |
Number of reflections | 52796 | 4463 |
<I/σ(I)> | 30.5 | 2.6 |
Completeness [%] | 99.6 | 98.8 |
Redundancy | 22.3 | 19.5 |
CC(1/2) | 0.990 | 0.560 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | Sodium malonate, MES |