7BRD

Crystal structure of Peptidyl-tRNA hydrolase from Klebsiella pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-12-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	70.203, 72.372, 77.415
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.208 - 1.892
Rwork	0.174
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pth
RMSD bond length	0.014
RMSD bond angle	1.792
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.970	1.970
High resolution limit [Å]	1.890	1.900
Rmerge	0.155	0.127
Rmeas	0.163	0.890
Rpim	0.049	0.271
Number of reflections	31852	31899
<I/σ(I)>	10.6
Completeness [%]	100.0	99.6
Redundancy	10.6	10.3
CC(1/2)	0.997	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.2M Ammonium Sulfate 0.1M Sodium cacodylate 30% PEG 8000